MK-2866 Ostarine 10 mg 60 capsules

One Peptides MK-2866 Ostarine – chemical reagent for research on the androgen receptor

Research reagent for laboratory use only. Not for human or veterinary use, diagnostic, or therapeutic purposes.

Batch-level quality documentation is available through the central HPLC/COA methodology & batch lookup page.

Related categories and reagents: In the context of this area of research, it is also worth comparing MK-677 Ibutamoren.

Ostarine (MK-2866) vs MK-677. Similar catalog codes, two different receptors. Ostarine (389.33 g/mol, CAS 841205-47-8) is a selective androgen receptor modulator (SARM). MK-677 (528.7 g/mol, CAS 159634-47-6) is a nonpeptide agonist of the ghrelin receptor GHSR1a, described in the context of the growth hormone axis; it is not associated with the androgen receptor. Comparing them as alternatives results from their proximity in price lists, not from a similarity of mechanism.

Introduction and legal framework

One Peptides MK-2866 Ostarine is a reference substance from the group of non-steroidal selective androgen receptor modulators. The description covers the chemical identity of the compound, the analytical quality of the material, the behaviour of the molecule in experimental models and the literature on the androgen receptor, myogenesis and bone metabolism.

In the literature, the compound is known as enobosarm, GTx-024 or ostarine. In this approach, MK-2866 appears in projects related to nuclear receptors, muscle cell differentiation, the pharmacokinetics of small-molecule performance reagents and the validation of LC-MS methods for research substances.

General characteristics of MK-2866

MK-2866 is a non-steroidal androgen receptor ligand. Unlike classic steroid androgens, it does not have a steroid skeleton. The core of the molecule is based on an arylpropanamide derivative with a trifluoromethyl moiety, two nitrile groups and a phenoxyamide fragment. This system affects the affinity for AR, lipophilicity and the behavior of the compound in chromatographic methods.

From a lab perspective, MK-2866 is interesting for several reasons. First, it has a well-described chemical identity and analytical profile. Second, it appears in work on myogenesis markers in the C2C12 and L6 lines. Third, it was evaluated in bone and pharmacokinetic models. Fourth, it is well suited for HPLC and LC-MS methods because it gives clear signals of the parent compound and metabolites.

What is MK-2866?

MK-2866 is a small molecule androgen receptor ligand with a chemical name (S)-N-(4-cyano-3-(trifluoromethyl)phenyl)-3-(4-cyanophenoxy)-2-hydroxy-2-methylpropanamide. In chemical documentation it is classified as a non-steroidal AR modulator. For the sake of precision, it is worth emphasizing that it is not a peptide and is not a prohormone. In the laboratory, it functions as a research substance for receptor, cellular and pharmacokinetic analyses.

Biochemical and identification data

This data set allows you to distinguish MK-2866 from other AR ligands and organize batch documentation before analysis. In laboratory practice, the CAS number, molar mass and PubChem record are the reference points for validating sample identity.

Structure and physicochemical properties of MK-2866

The structure of MK-2866 includes an aromatic phenoxy system, an amide moiety, a stereogenic center and a hydroxyl group. The presence of the trifluoromethyl fragment increases lipophilicity and affects the interaction with the hydrophobic pocket of the androgen receptor. At the same time, the amide and hydroxyl groups participate in the formation of hydrogen bonds that stabilize the ligand-receptor complex.

In reference materials, MK-2866 is usually described as a white or off-white solid. From the point of view of the laboratory, it is important that the compound behaves better in organic systems than in highly aqueous media. This is of direct importance when preparing working solutions for receptor assays, LC-MS, and cell projects.

Solubility and sample preparation

Available catalog data and research materials indicate good compatibility of MK-2866 with DMSO, DMF and ethanol. In more aqueous environments, especially at a pH close to neutral, the solubility is clearly lower. For this reason, laboratories typically prepare the stock solution in an organic solvent and then dilute it into the experimental medium while maintaining control over the risk of precipitation.

Before starting the experiment, it is worth confirming the stability of the sample in the selected matrix and checking that the excipient does not interfere with the analytical reading. This is especially important in cell tests, where even a small change in solvent can affect the result regardless of MK-2866 itself.

Purity, identification and quality control

In laboratory tests, the name of the compound itself does not give credibility. The quality of the material is determined by batch documentation and consistency of results from analytical methods. In the case of MK-2866, it is particularly important to combine chromatographic data with molecular weight measurement and assessment of sample homogeneity.

HPLC

High performance liquid chromatography is used to assess chromatographic uniformity. For MK-2866, HPLC analysis allows you to check whether the dominant peak corresponds to the expected substance and whether the level of impurities is within acceptable limits for a given protocol. In the case of material prepared for quantitative research, such control is the first quality filter.

LC-MS or HRMS

Mass spectrometry confirms the molecular weight and fragmentation pattern. In the case of MK-2866, LC-MS/MS methods are particularly useful because they can track both the parent compound and metabolites that appear in ADME projects. This approach facilitates validation of material identity and comparison of results between test series.

Storage and laboratory stability

For the MK-2866 reference material, a dry, cool and light-free environment is most often assumed, usually around -20°C. After preparing the working solution, it is worth working with small aliquots to limit repeated thawing and concentration fluctuations. It is a good idea to note the date of preparation, the solvent used and the storage conditions in the batch documentation.

Research context and laboratory endpoints

The best described areas of application for MK-2866 include AR research, muscle cell differentiation projects, bone models, and pharmacokinetic experiments. In this sense, the compound is a tool for analyzing androgen receptor-dependent pathways, not a consumer product.

Androgen receptor and transcription

MK-2866 binds to the ligand-binding domain of the androgen receptor, causing conformational changes leading to the activation of AR-dependent genes. For laboratories studying nuclear receptors, this provides a useful model for observing receptor translocation, coactivator recruitment, and changes in the expression of markers associated with the androgen response.

Myogenesis in cell lines

In publications covering the C2C12 and L6 lines, changes in markers such as MyoD, myogenin and MyH were observed after exposure to enobosarm. These types of results are used to analyze muscle cell differentiation and the relationship between AR and ERK1/2 pathways.

Bone tissue models

MK-2866 also appears in bone research, where the microstructure, remodeling rate and behavior of bone material were analyzed in animal models. The interpretation of the results here depends on the exposure scheme, observation time and model used, so this area requires very close control of the protocol.

Pharmacokinetics and metabolites

ADME data for the rat model demonstrate the broad distribution, presence of metabolites, and suitability of MK-2866 in LC-MS designs. This makes the compound useful not only in mechanistic studies, but also in projects focused on metabolite identification, matrix recovery and analyte stability.

Comparison with other reagents from the same group

If the goal of the project is to analyze the impact of PPARδ activation on performance and metabolism, it is worth considering GW-501516 reagents. When the priority is the GHS-R1a axis and GH/IGF markers, MK-677 reagents remain a reasonable reference point. In projects focusing on the circadian clock and Rev-Erb pathways, results can be additionally cross-referenced with data for SR-9009 reagents.

Related research: SARMs research — what the science says · Ostarine (MK-2866) — state of the research · MK-2866 research reagents