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Product quality tests using the HPLC method revealing the concentration and purity of the tested reagents
Peptides created with precision and quality in mind
We offer peptides for research applications, created based on current scientific knowledge and rigorous quality control standards. Our products combine high purity, precisely defined composition and excellent repeatability of parameters, ensuring reliable and reproducible results.
Peptides are widely used in research on regenerative processes, tissue functioning, cell signaling and biological mechanisms at the molecular level. Each batch is subjected to detailed laboratory analyses to confirm compliance with the technical specifications.
By choosing our products, you can be sure of working with materials of proven quality, stability and laboratory safety – designed with the requirements of modern research and scientific teams in mind.
Quality testing, HPLC and One Peptides certificates
Each batch of peptide and each batch of SARM capsules leaving our warehouse is assigned an analytical document. This page explains what quality control looks like, what an HPLC chromatogram shows, what you’ll find in a certificate of analysis (COA), and how to match the document to a specific batch of product.
The scope of documents includes research peptides in lyophilized form (Research Use Only reagents), capsule-format RUO reagents from the performance range (including SARMs and related research compounds), and other reagents in capsule and solution form. Each product framework has its own regulatory context, but all documented batches follow a common analytical standard. Some of the technical topics – reconstitution, aliquoting, cold chain – are discussed in more detail in the guide Laboratory practice with research peptides. Descriptions of molecular mechanisms and summaries of scientific evidence can be found on product pages and in the blog section.
What does the quality control of each batch look like?
Each batch goes through a three-step process before being offered for sale.
Lot identification. After accepting the raw material from the GMP-equivalent synthesis, the batch receives a unique batch number and production date. The number remains with the product until the end of the distribution cycle – it appears on the vial label, invoice and certificate of analysis.
Laboratory analysis and verification. Each batch is tested using high-performance liquid chromatography (HPLC) with UV detection. For peptides, we add mass spectrometry (MS) identity confirmation, which verifies the molecular weight against the theoretical one. Reference samples are kept for retest. The results are then compared with the product specification sheet: minimum purity ≥98%, allowable molar mass deviation, water content limits (Karl Fischer for selected items), residual solvent limits. A batch that does not meet specifications will not be sold.
Certificate of analysis and release for sale. After positive verification, we issue a certificate of analysis (COA), which we publish as a PDF file available from the quality page and the product page. The cold chain (2-8°C) applies from the moment of COA issuance until final delivery.
What does HPLC analysis show?
Liquid chromatography separates a mixture of compounds by passing it through a column with a stationary phase. Each component moves at a different speed and leaves the column at different times (retention time). The UV detector records the concentration of compounds, and the result is presented as a chromatogram – a graph of absorbance versus time.
Peaks appear in the chromatogram. The main peak is the peptide or active substance being tested. Side peaks are impurities: synthesis by-products, degraded forms, trace amounts of reagents.
What ≥98% purity means. The area under the main peak is at least 98% of the sum of the areas of all peaks recorded in the chromatogram. It is a measure of percentage, not a measure of biological activity or absolute mass.
What does compliance mean? The retention time of the main peak corresponds to the reference standard (±method tolerance) and the chromatogram profile does not show any impurity peaks exceeding the established limits.
What HPLC cannot resolve on its own. The method confirms chromatographic purity and the presence of the compound at the appropriate retention time, but does not clearly confirm the identity of the chemical structure. That’s why we add mass spectrometry for peptides – the molecular weight recorded in MS is a separate, independent proof of identity. HPLC also does not assess biological activity or physiological effects on a living organism.
What does a Certificate of Analysis (COA) contain?
Each certificate of analysis includes a fixed set of information fields. Thanks to this, you can compare one document with another and independently verify that the batch complies with the specifications.
| COA field | Description |
|---|---|
| Product name | Full chemical name and symbol (e.g. BPC-157, Thymosin Beta-4 Fragment 17-23 / TB-500) |
| Batch No. | Unique lot identifier – the same number appears on the vial label |
| Analysis date | The day the HPLC and MS test was performed |
| Expiry date/retest | Date until which compliance with the specification is valid under storage conditions |
| Test method | HPLC-UV; for peptides additionally MS (ESI-MS or MALDI-TOF, depending on the product) |
| Purity result | Percentage of main peak contribution (e.g. ≥98.4%) |
| Molecular weight | Value measured in MS relative to theoretical value |
| Storage conditions | Temperature, protection from light, humidity |
| PDF file | The full document with the chromatogram and MS spectrum is attached |
| Related product page | Link to the product in the store the lot is associated with |
How do you match the certificate to the product and batch number?
Each peptide vial or SARM blister is labeled with a batch number. The verification procedure is simple.
- Check the batch number on the label of the product you received (Batch No. / Lot No.).
- Go to the quality page or the product page in the store.
- Download the COA file corresponding to your lot number.
- Compare the data on the vial (name, quantity, date) with the fields in the certificate.
If the lot number on a product does not match any published COA, please email us via the contact page — we respond within one business day with the document for your batch.
What the quality certificate does not confirm
The certificate of analysis confirms the parameters measured in the laboratory for the tested batch at a specific point in time. Anything beyond that is beyond the scope of the document.
- COA is not a declaration of effectiveness. A purity of 98% indicates the content of the main compound in the sample, not what effect this compound will have in any research model.
- A COA is not a marketing authorisation for a medicine. All items in our catalog are chemical reagents intended exclusively for laboratory research (Research Use Only). They are not medicinal products, they are not dietary supplements, they are not foodstuffs.
- COA is not a substitute for risk assessment. The decision on how to use a reagent in a laboratory protocol rests with the researcher. The document does not exempt you from the need to follow safety rules while working in the laboratory.
- COA does not cover stability after reconstitution. The result applies to the freeze-dried form under storage conditions consistent with the specifications. After dissolving in bacteriostatic water the stability period depends on the storage conditions of the solution – this is a separate parameter.
- The scope of documentation depends on the form of the reagent. For lyophilized reagents, it includes HPLC purity, identity confirmation by MS, and a certificate of analysis for the batch. For reagents in capsule and solution form (including BPC-157 in capsules, Yohimbine, L-carnitine), the scope may be narrower: where batch documentation is available, we publish scans on the product page.
Frequently asked questions about HPLC, COA and certificates
Does each product have a separate certificate?
Yes. Each batch of each product receives its own COA associated with the batch number. Two different lots of the same peptide have two separate documents.
Can I check the lot number before purchasing?
The number of the batch currently being shipped is available on the product page in the quality section. If you are planning a purchase for specific documentation, contact customer service via the contact form – we will confirm which batch will be included in your shipment.
Does HPLC confirm both purity and identity?
HPLC independently confirms chromatographic purity and compliance of the retention time with the standard. For peptides, the structural identity is additionally confirmed by mass spectrometry (MS) – these are two independent methods in one document.
How often are documents updated?
The COA is updated with each new batch. When the old batch runs out, the new one receives a fresh certificate with the current analysis date. Earlier documents remain available in the archive for people who purchased earlier batches.
Related materials
Reconstitution of lyophilized reagents is supported by the peptide calculator, which helps calculate the volume of bacteriostatic water and the concentration of the working solution.
The full range with quality documentation is available in the shop, divided into research peptides and performance products in capsules. Representative items with published COAs:
- BPC-157 10 mg — pentadecapeptide, best documented in the regenerative literature (animal models)
- TB-500 10 mg — fragment of Thymosin Beta-4, studied in the context of cell proliferation
- Selank 10 mg — heptapeptide tested in neuropharmacological models
- Epithalon 10 mg — tetrapeptide in longevity studies
- MK-677 (Ibutamoren) 60 capsules — GHS-R1a receptor agonist (not a SARM), RUO reagent in capsule form
