DSIP 5 mg

One Peptides DSIP 5 mg (Delta Sleep Inducing Peptide) – chemical reagent for laboratory tests

DSIP – Research Use Only (RUO). Chemical reagent intended only for laboratory tests. It is not intended for human consumption or diagnostic purposes.
Related categories and reagents: In the context of this area of ​​research, it is also worth comparing Sleepwell.

DSIP vs SleepWell. DSIP is a single, defined nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu (C35H48N10O15, 848.81 g/mol, CAS 62568-57-4). SleepWell is a multicomponent preparation containing, per 1 mL, 50 mg GABA, 50 mg L-arginine, 14 mg glutamine and 1 mg melatonin—a declared total of 115 mg/mL. Structurally, these are three free amino acids and one indoleamine; none of the SleepWell ingredients is a peptide. The difference is categorical: one defined molecule with full analytical characterization compared with a mixture of four small molecules. In an experiment, this determines whether an observation can be attributed to a specific substance or only to the preparation as a whole.

Introduction and research framework

DSIP (Delta Sleep Inducing Peptide) is a naturally occurring neuropeptide consisting of 9 amino acids, which was first isolated in 1977 by Swiss scientists from the blood of rabbits during induced sleep. Since its discovery, DSIP has become the subject of intense neurobiological research due to its unique molecular properties and presence in the central nervous system of mammals.

The DSIP peptide is studied in the context of a variety of neurobiological processes, including the regulation of circadian rhythms, modulation of neurotransmission, and interaction with the hypothalamic-pituitary axis. Its relatively simple structure and well-understood sequence make it a valuable tool in neuroscience research.

General characteristics of the compound

DSIP (Delta Sleep Inducing Peptide) is a nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, characterized by a unique structure containing a phosphorylated serine residue at position 7 in the native form. This peptide occurs endogenously in the mammalian brain, where it has been identified in the hypothalamus, pituitary gland and other limbic structures.

The name “Delta Sleep Inducing Peptide” comes from observations from early studies in which the peptide was isolated during the phase of sleep characterized by delta waves in the EEG recording. However, modern research indicates a much wider range of potential functions of this neuropeptide in the central nervous system.

In the context of laboratory research, DSIP is used as a tool to study neuropeptide mechanisms, interactions with receptors and molecular processes in the nervous system.

What is DSIP?

DSIP is an endogenous neuropeptide found in mammals, the presence of which has been confirmed in:

• Hypothalamus
• Pituitary gland
• Cerebrospinal fluid
• Blood plasma
• Other peripheral tissues

History of Discovery

DSIP forms

Structure and physicochemical properties

Amino Acid Sequence

DSIP consists of 9 amino acids arranged in the following sequence:

H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH

One letter code: WAGGDASGE

Sequence Characteristics

• Position 1 (Trp) – tryptophan, an aromatic amino acid
• Positions 2, 6 (Ala) – alanine, a small hydrophobic amino acid
• Positions 3, 4, 8 (Gly) – glycine, gives flexibility to the chain
• Position 5 (Asp) – aspartic acid, an acidic amino acid
• Position 7 (Cheese) – serine, site of potential phosphorylation
• Position 9 (Glu) – glutamic acid, C-terminus

Physicochemical properties

Spectral Properties

• UV Absorption: Maximum at 280 nm (tryptophan)
• Fluorescence: Emission at ~350 nm (excitation 280 nm)
• Characteristic MS ions: [M+H]+ = 849.8 m/z

Purity, identification and quality control

Product Specification ONE DSIP 5 mg

Quality parameters:

Storage and laboratory handling

Storage Conditions

Freeze-dried form:
– Temperature: -20°C (optimal)
– Permissible: 2-8°C (short term)
– Protect from light (tryptophan sensitive to UV)
– Store in a dry place
– Shelf life: 24 months at -20°C

After reconstitution:
– Solvent: sterile water or PBS
– Storage: 2-8°C
– Use within 7 days
– Freeze aliquots for longer storage
– Avoid repeated freezing/thawing

Reconstitution Instructions

1. Equalize the temperature of the vial (15-20 min)
2. Add sterile water slowly along the side
3. Recommended concentration: 1-5 mg/ml
4. Mix gently by inverting the vial
5. Protect the solution from light
6. Store in the refrigerator or freeze

Mechanism of action at the molecular level

Molecular Interactions

DSIP is studied in the context of various molecular mechanisms. It should be emphasized that the information below comes from laboratory tests and does not constitute claims of effect in humans.

Potential molecular targets (in vitro studies):

1. Opioid receptors

• Studies on the interaction with μ and δ receptors
• Modulation of endorphin binding

2. GABA receptors

• Potential modulation of GABAergic transmission
• Interactions with the GABA-A receptor complex

3. Glutamatergic system

• Research on NMDA receptors
• Effect on excitatory transmission

4. Stress hormones

• Interactions with the HPA axis in cellular models
• Modulation of CRH/ACTH secretion

Research on Cell Cultures

The literature describes DSIP research on:

• Neurons cultured in vitro
• Pituitary cells
• Neuroblastoma cell lines
• Brain tissue preparations

DSIP phosphorylation

Serine at position 7 can be phosphorylated:

• pDSIP (phospho-DSIP) as the active form
• Studies on the role of phosphorylation in activity
• Phosphorylation/dephosphorylation kinetics

Applications in Scientific Research

Neurobiology

• Study of neuropeptide mechanisms
• Modeling peptide-receptor interactions in the CNS
• Research on circadian rhythms
• Analysis of signaling pathways in neurons

Biochemical Research

• Characteristics of binding to receptors
• Structure-activity studies
• Stability analysis in various conditions
• Peptide-membrane interactions

Chronobiology

• Research on the biological clock
• The effect of peptides on circadian rhythms (in vitro models)
• Clock gene expression

Comparative Research

• DSIP vs. other sleep neuropeptides
• Comparison of phosphorylated and unphosphorylated forms
• Studies of interspecies differences

Reservations

IMPORTANT: The product is intended for in vitro testing only. Not intended for:
– Use in humans or animals
– Diagnostic or therapeutic purposes
– Any clinical use

Summary

DSIP (Delta Sleep Inducing Peptide) is a naturally occurring nonapeptide that is a valuable tool in neurobiological and biochemical research. Its simple structure, presence in the central nervous system of mammals, and well-understood chemical properties make it a useful reagent in neuropeptide research.

One Peptides DSIP 5 mg material offers:

• High purity ≥98% HPLC
• Full spectrometric characterization
• Stable freeze-dried form
• Certificate of analysis for each batch

Science FAQ

1. What is DSIP and what is its structure?

DSIP (Delta Sleep Inducing Peptide) is a nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, consisting of 9 amino acids with a molar mass of 848.81 g/mol. It is an endogenous neuropeptide found in mammals, first isolated in 1977 from the blood of rabbits. The CAS number is 62568-57-4.

2. Where does DSIP occur naturally?

DSIP has been identified in various mammalian tissues, including the hypothalamus, pituitary gland, cerebrospinal fluid, and blood plasma. It is an endogenous neuropeptide whose presence has also been confirmed in human tissues.

3. How to properly store DSIP?

In freeze-dried form, DSIP should be stored at -20°C, protected from light (tryptophan is sensitive to UV). After reconstitution in sterile water or PBS, the solution is stored at 2-8°C and used within 7 days. For longer storage, it is recommended to freeze small portions.

4. What is the difference between DSIP and pDSIP?

pDSIP is a phosphorylated form of DSIP in which the serine residue at position 7 is phosphorylated (Ser-7-PO4). Phosphorylation may affect the biological properties of the peptide. The standard DSIP product is the unphosphorylated form.

5. What research is DSIP used for?

DSIP is used in in vitro research in the field of neurobiology, biochemistry and chronobiology. Applications include: the study of neuropeptide mechanisms, interactions with receptors in the CNS, structure-activity studies, and modeling of circadian rhythms. It is not intended for use on humans or animals.

The most important research conclusions

The most important features of the ONE DSIP 5 mg product:

• Type – endogenous neuropeptide (nonapeptide)
• Sequence – WAGGDASGE (9 amino acids)
• Molar mass – 848.81 g/mol
• CAS number – 62568-57-4
• Cleanliness – minimum 98% HPLC
• Form – lyophilisate (white powder)
• Property – contains tryptophan (fluorescent)
• Destiny – only in vitro tests