Buy Kisspeptin-10 5 mg research vial (RUO)

Name: KISSPEPTIN-10 5MG
Brand: One-Peptides
SKU: OP-000021
Price: 35 EUR
Availability: InStock

One Peptides Kisspeptin-10 5 mg – chemical reagent for laboratory tests

Kisspeptin – Research Use Only (RUO). Chemical reagent intended only for laboratory tests. It is not intended for human consumption or diagnostic purposes.
Related categories and reagents: In the context of this area of research, it is also worth comparing Oxytocin 10 mg.

Kisspeptin-10 vs oxytocin. Both are short peptides described in the context of neuroendocrinology, but they enter two different receptor systems. Kisspeptin-10 (1,302.45 g/mol) is a decapeptide and a ligand of the KISS1R receptor (formerly GPR54), described in the literature in the context of regulating the hypothalamic-pituitary-gonadal axis. Oxytocin (1,007.19 g/mol) is a cyclic nonapeptide and a ligand of OXTR, which belongs to a completely different receptor family. The research area is shared, not the mechanism; these are not interchangeable reagents.

Introduction and research framework

Kisspeptin-10 (Kp-10), also known as metastin 45-54, is a synthetic decapeptide that is the C-terminal fragment of full-molecule kisspeptin-54. This peptide is a biologically active fragment of the KISS1 gene product, which plays a central role in the regulation of the hypothalamic-pituitary-gonadal (HPG) axis by activating the KISS1R receptor (GPR54).

The discovery of kisspeptin in 2003 was a breakthrough in understanding the mechanisms of reproductive function control at the neuroendocrine level. Kisspeptin-10 retains the full biological activity of full-molecule kisspeptin, which makes it a valuable tool in research on the regulation of gonadotropin secretion.

General characteristics of the compound

Kisspeptin-10 is a decapeptide with the sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2, corresponding to positions 112-121 (or 45-54 counting from the C-terminus) of the kisspeptin precursor. This is the minimal sequence that retains full affinity for the KISS1R receptor and biological activity.

In scientific research, Kisspeptin-10 serves as:

A tool for examining the function of the KISS1R receptor
Model in research on the HPG axis
Reference ligand in neuroendocrine research
Standard in kisspeptin structure-activity studies

What is Kisspeptin-10?

Kisspeptin-10 is a C-terminal decapeptide that is the active fragment of kisspeptin-54 (metastin). The KISS1 gene encodes a 145 amino acid precursor that is proteolytically processed into active forms: kisspeptin-54, -14, -13 and -10.

History of Discovery

Year	Event
1996	Discovery of the KISS1 gene as a metastasis suppressor
2001	Identification of the GPR54 receptor (KISS1R)
2003	Discovery of the role of kisspeptin in GnRH regulation
2003	Association of KISS1R mutations with hypogonadism
2005+	Intensive research on reproductive functions

Origin of the Name

The name “kisspeptin” comes from the place where the KISS1 gene was discovered – Hershey in Pennsylvania (USA), known for the production of Hershey’s Kisses chocolates. The alternative name “metastin” refers to initial research into its role in suppressing cancer metastases.

Kisspeptin forms

Form	Length	Positions	Activity
Kisspeptin-54	54 A.A	68-121	Full
Kisspeptin-14	14 A.A	108-121	Full
Kisspeptin-13	13 A.A	109-121	Full
Kisspeptin-10	10 A.A	112-121	Full

Structure and physicochemical properties

Amino Acid Sequence

Kisspeptin-10 consists of 10 amino acids:

H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2

One letter code: YNWNSFGLRF-NH2

Characteristics of Amino Acids

Position 1 (Tyre) – tyrosine, aromatic, N-terminus
Position 2 (Asn) – asparagine, polar
Position 3 (Trp) – tryptophan, aromatic (key)
Position 4 (Asn) – asparagine, polar
Position 5 (Cheese) – serine, polar
Position 6 (Phe) – phenylalanine, aromatic
Position 7 (Gly) – glycine
Position 8 (Leu) – leucine, hydrophobic
Position 9 (Arg) – arginine, basic
Position 10 (Phe) – phenylalanine, C-terminus amidated (key)

Physicochemical properties

Parameter	Value
Summary formula	C63H83N17O14
Molar mass	1302.45 g/mol
CAS number	374675-21-5
Number of amino acids	10
C-end	Amidated (-NH2)
Isoelectric point (pI)	~10.0 (basic)
Solubility	Soluble in water
Form	Freeze-dried powder (white)
Aromatic amino acids	4 (Tyr, Trp, 2×Phe)

RF-amide motif

Kisspeptin-10 belongs to the RF-amide peptide family, characterized by the C-terminal sequence Arg-Phe-NH2:

A conserved motif in vertebrates
Crucial for binding to the receptor
Common feature with other RF-amide peptides (RFRP, NPFF)

Purity, identification and quality control

Product Specification ONE Kisspeptin-10 5 mg

Quality parameters:

Test	Method	Specification
Cleanliness	HPLC	≥98%
Identification	MS	1302.45 ± 0.5 Da
Water content	Karl Fischer	≤8%
Acetate content	Titration	≤12%
Appearance	Visual	White lyophilisate
Solubility	Test	Clear in water

Storage and laboratory handling

Storage Conditions

Freeze-dried form:
– Temperature: -20°C (optimal)
– Permissible: 2-8°C (up to 1 month)
– Protect from light (tryptophan!)
– Protect against moisture
– Shelf life: 24 months at -20°C

After reconstitution:
– Solvent: sterile water or PBS
– Storage: 2-8°C in the dark
– Use within 7-14 days
– Freezing aliquots recommended
– Avoid repeated freezing/thawing

Reconstitution Instructions

Equalize the temperature of the vial (15-20 min)
Add sterile water slowly along the side
Recommended concentration: 0.5-2 mg/ml
Mix gently by turning
Protect the solution from light
Store in the refrigerator or freeze

Notes on Stability

Tryptophan at position 3 is sensitive to UV light
Asparagines may undergo deamidation
Store in the dark
Avoid long-term storage in solution

Mechanism of action at the molecular level

KISS1R receptor (GPR54)

Kisspeptin-10 is an agonist of the KISS1R receptor, a Gq/11 protein-coupled GPCR. In vitro tests analyze:

Binding characteristics:
– High affinity for KISS1R
– EC50 in the nanomolar range
– Comparison with longer forms of kisspeptin

Intracellular signaling:
– Activation of phospholipase C
– IP3 and DAG production
– Release of Ca2+ from the ER
– Activation of PKC and MAPK

Structure-Activity Research

In vitro studies have established:

C-terminal Phe-NH2 necessary for activity
Trp-3 important for affinity
N-terminal truncation tolerated up to Kp-10
Further shortening reduces activity

Research on Cell Cultures

Kisspeptin-10 has been tested for:

GnRH-producing cells (GT1-7, GnRH neurons)
Lines expressing KISS1R
Gonadotropic cells of the pituitary gland
Hypothalamic neurons

Interactions with Other Systems

In vitro studies show potential interactions with:

GnRH neurons
The opioid system
Stress neuropeptides
Metabolic pathways

Applications in Scientific Research

Neuroendocrinology

HPG axis adjustment mechanisms
Control of GnRH secretion
Integration of metabolic and reproductive signals
Pulsatility of gonadotropin secretion

Receptor Research

KISS1R/GPR54 characteristics
Structure-activity studies of kisspeptins
Selectivity and specificity
Receptor desensitization

Reproductive Biology

Mechanisms of initiation of puberty
Control of the sexual cycle
Seasonality of reproduction in animals
Photoperiodic integration

Metabolic Research

Connections between metabolism and reproduction
Effect of energy state on KISS1R
Interactions with leptin and insulin

Reservations

IMPORTANT: The product is intended for in vitro testing only. Not intended for:
– Use in humans or animals
– Diagnostic or therapeutic purposes
– Any clinical use

Summary

Kisspeptin-10 is a synthetic decapeptide that is the minimal active fragment of kisspeptin, retaining full affinity for the KISS1R receptor. Its central role in regulating the HPG axis makes it an indispensable tool in neuroendocrine research and reproductive biology.

Material One Peptides Kisspeptin-10 5 mg offers:

High purity ≥98% HPLC
Full spectrometric characterization
Stable freeze-dried form
Certificate of analysis for each batch

Science FAQ

1. What is Kisspeptin-10 and what is its structure?

Kisspeptin-10 (Kp-10) is a synthetic decapeptide with the sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 (YNWNSFGLRF-NH2) and a molar mass of 1302.45 g/mol. This is the C-terminal fragment of kisspeptin-54, retaining full activity towards the KISS1R receptor. The CAS number is 374675-21-5.

2. What is the role of kisspeptin in the body?

Kisspeptin is a key regulator of the hypothalamic-pituitary-gonadal (HPG) axis. Kisspeptin neurons in the hypothalamus control the secretion of GnRH, which in turn regulates the secretion of gonadotropins (LH, FSH) from the pituitary. Mutations in the KISS1 or KISS1R gene lead to puberty disorders.

3. How to store Kisspeptin-10?

In lyophilized form, the peptide is stored at -20°C, protected from light (due to tryptophan) and moisture. After reconstitution in sterile water, the solution is stored at 2-8°C for up to 7-14 days in the dark. It is recommended to freeze small portions for longer storage.

4. What is the RF-amide motif and why is it important?

The RF-amide motif is the C-terminal Arg-Phe-NH2 (RF-NH2) sequence, characteristic of the RF-amide peptide family to which kisspeptin belongs. This motif is necessary for binding to the KISS1R receptor – its modification leads to the loss of the biological activity of the peptide.

5. What tests is Kisspeptin-10 used for?

Kisspeptin-10 is used in in vitro studies in the field of: neuroendocrinology (regulation of the HPG axis), reproductive biology, KISS1R receptor studies, GnRH control mechanisms and integration of metabolic and reproductive signals. It is not intended for use on humans or animals.

The most important research conclusions

The most important features of the ONE Kisspeptin-10 5 mg product:

Type – neuropeptide, regulator of the HPG axis
Sequence – YNWNSFGLRF-NH2 (10AA)
Molar mass – 1302.45 g/mol
CAS number – 374675-21-5
Receptor – KISS1R (GPR54)
Family – RF-amide peptides
Cleanliness – minimum 98% HPLC
Form – lyophilisate (white powder)
Destiny – only in vitro tests

Bibliography

de Roux N, et al. (2003) “Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54” – Proceedings of the National Academy of Sciences USA, 100(19):10972-10976.
Link: https://pubmed.ncbi.nlm.nih.gov/12944565/
Seminara SB, et al. (2003) “The GPR54 gene as a regulator of puberty” – New England Journal of Medicine, 349(17):1614-1627.
Link: https://pubmed.ncbi.nlm.nih.gov/14573733/
Kotani M, et al. (2001) “The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54” – Journal of Biological Chemistry, 276(37):34631-34636.
Link: https://pubmed.ncbi.nlm.nih.gov/11457843/