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HPLC vs MS – methods for analyzing the purity of research peptides
In a laboratory working with peptides, there comes a moment when the supplier sends a certificate of analysis with two key data: an HPLC chromatogram with the declaration “purity ≥98%” and a mass spectrum with the molecular mass consistent with the theoretical value. To a person without a background in analytical chemistry, both documents look like a technical formality – a strip of peaks, a few numbers, the signature of a QC employee. […]
