{"id":1017,"date":"2024-11-23T08:19:02","date_gmt":"2024-11-23T08:19:02","guid":{"rendered":"https:\/\/one-peptides.com\/product\/dsip-5mg\/"},"modified":"2026-10-09T08:04:28","modified_gmt":"2026-10-09T08:04:28","slug":"dsip-5mg","status":"publish","type":"product","link":"https:\/\/one-peptides.com\/de\/produkt\/dsip-5mg\/","title":{"rendered":"DSIP 5 mg"},"content":{"rendered":"<h2><strong>One Peptides DSIP 5 mg (Delta Sleep Inducing Peptide) &#8211; chemical reagent for laboratory tests<\/strong><\/h2>\n<p><span style=\"font-weight: 400;\">DSIP &#8211; Research Use Only (RUO). Chemical reagent intended only for laboratory tests. It is not intended for human consumption or diagnostic purposes.<\/span><br \/>\n<strong>Related categories and reagents:<\/strong> In the context of this area of \u200b\u200bresearch, it is also worth comparing <a href=\"https:\/\/one-peptides.com\/product\/sleepwell-115mg-ml\/\">Sleepwell.<\/a><\/p>\n<p><strong>DSIP vs SleepWell.<\/strong> DSIP is a single, defined nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu (C35H48N10O15, 848.81 g\/mol, CAS 62568-57-4). SleepWell is a multicomponent preparation containing, per 1 mL, 50 mg GABA, 50 mg L-arginine, 14 mg glutamine and 1 mg melatonin\u2014a declared total of 115 mg\/mL. Structurally, these are three free amino acids and one indoleamine; <strong>none of the SleepWell ingredients is a peptide.<\/strong> The difference is categorical: one defined molecule with full analytical characterization compared with a mixture of four small molecules. In an experiment, this determines whether an observation can be attributed to a specific substance or only to the preparation as a whole.<\/p>\n<h2><strong>Introduction and research framework<\/strong><\/h2>\n<p>DSIP (Delta Sleep Inducing Peptide) is a naturally occurring neuropeptide consisting of 9 amino acids, which was first isolated in 1977 by Swiss scientists from the blood of rabbits during induced sleep. Since its discovery, DSIP has become the subject of intense neurobiological research due to its unique molecular properties and presence in the central nervous system of mammals.<\/p>\n<p>The DSIP peptide is studied in the context of a variety of neurobiological processes, including the regulation of circadian rhythms, modulation of neurotransmission, and interaction with the hypothalamic-pituitary axis. Its relatively simple structure and well-understood sequence make it a valuable tool in neuroscience research.<\/p>\n<h2><strong>General characteristics of the compound<\/strong><\/h2>\n<p>DSIP (Delta Sleep Inducing Peptide) is a nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, characterized by a unique structure containing a phosphorylated serine residue at position 7 in the native form. This peptide occurs endogenously in the mammalian brain, where it has been identified in the hypothalamus, pituitary gland and other limbic structures.<\/p>\n<p>The name &#8220;Delta Sleep Inducing Peptide&#8221; comes from observations from early studies in which the peptide was isolated during the phase of sleep characterized by delta waves in the EEG recording. However, modern research indicates a much wider range of potential functions of this neuropeptide in the central nervous system.<\/p>\n<p>In the context of laboratory research, DSIP is used as a tool to study neuropeptide mechanisms, interactions with receptors and molecular processes in the nervous system.<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-1142 size-large\" src=\"https:\/\/one-peptides.com\/wp-content\/uploads\/2025\/07\/Dip-Camera-e1751211927410-1024x722.png\" alt=\"DSIP na sen\" width=\"800\" height=\"564\" \/><\/p>\n<h2><strong>What is DSIP?<\/strong><\/h2>\n<p>DSIP is an endogenous neuropeptide found in mammals, the presence of which has been confirmed in:<\/p>\n<ul>\n<li>Hypothalamus<\/li>\n<li>Pituitary gland<\/li>\n<li>Cerebrospinal fluid<\/li>\n<li>Blood plasma<\/li>\n<li>Other peripheral tissues<\/li>\n<\/ul>\n<h3><strong>History of Discovery<\/strong><\/h3>\n<table>\n<thead>\n<tr>\n<th>Year<\/th>\n<th>Event<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>1977<\/td>\n<td>Isolation of DSIP from rabbit blood (Schoenenberger, Monnier)<\/td>\n<\/tr>\n<tr>\n<td>1977<\/td>\n<td>Determination of the amino acid sequence<\/td>\n<\/tr>\n<tr>\n<td>1980s<\/td>\n<td>Intensive research on physiological functions<\/td>\n<\/tr>\n<tr>\n<td>1990s<\/td>\n<td>Identification in human tissues<\/td>\n<\/tr>\n<tr>\n<td>2000s+<\/td>\n<td>Research on molecular mechanisms<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><strong>DSIP forms<\/strong><\/h3>\n<table>\n<thead>\n<tr>\n<th>Form<\/th>\n<th>Description<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>DSIP<\/td>\n<td>Standard unphosphorylated form<\/td>\n<\/tr>\n<tr>\n<td>pDSIP<\/td>\n<td>Phosphorylated form (Ser-7-PO4)<\/td>\n<\/tr>\n<tr>\n<td>DSIP-OH<\/td>\n<td>C-terminus free<\/td>\n<\/tr>\n<tr>\n<td>DSIP-NH2<\/td>\n<td>C-terminus amidated<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2><strong>Structure and physicochemical properties<\/strong><\/h2>\n<h3><strong>Amino Acid Sequence<\/strong><\/h3>\n<p>DSIP consists of 9 amino acids arranged in the following sequence:<\/p>\n<p><strong>H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH<\/strong><\/p>\n<p>One letter code: <strong>WAGGDASGE<\/strong><\/p>\n<h3><strong>Sequence Characteristics<\/strong><\/h3>\n<ul>\n<li><strong>Position 1 (Trp)<\/strong> \u2013 tryptophan, an aromatic amino acid<\/li>\n<li><strong>Positions 2, 6 (Ala)<\/strong> \u2013 alanine, a small hydrophobic amino acid<\/li>\n<li><strong>Positions 3, 4, 8 (Gly)<\/strong> \u2013 glycine, gives flexibility to the chain<\/li>\n<li><strong>Position 5 (Asp)<\/strong> \u2013 aspartic acid, an acidic amino acid<\/li>\n<li><strong>Position 7 (Cheese)<\/strong> \u2013 serine, site of potential phosphorylation<\/li>\n<li><strong>Position 9 (Glu)<\/strong> \u2013 glutamic acid, C-terminus<\/li>\n<\/ul>\n<h3><strong>Physicochemical properties<\/strong><\/h3>\n<table>\n<thead>\n<tr>\n<th>Parameter<\/th>\n<th>Value<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Summary formula<\/td>\n<td>C35H48N10O15<\/td>\n<\/tr>\n<tr>\n<td>Molar mass<\/td>\n<td>848.81 g\/mol<\/td>\n<\/tr>\n<tr>\n<td>CAS number<\/td>\n<td>62568-57-4<\/td>\n<\/tr>\n<tr>\n<td>Number of amino acids<\/td>\n<td>9<\/td>\n<\/tr>\n<tr>\n<td>Isoelectric point (pI)<\/td>\n<td>~3.8 (acidic)<\/td>\n<\/tr>\n<tr>\n<td>Solubility<\/td>\n<td>Soluble in water<\/td>\n<\/tr>\n<tr>\n<td>Form<\/td>\n<td>Freeze-dried powder (white)<\/td>\n<\/tr>\n<tr>\n<td>Stability<\/td>\n<td>Stable in freeze-dried form<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><strong>Spectral Properties<\/strong><\/h3>\n<ul>\n<li><strong>UV Absorption:<\/strong> Maximum at 280 nm (tryptophan)<\/li>\n<li><strong>Fluorescence:<\/strong> Emission at ~350 nm (excitation 280 nm)<\/li>\n<li><strong>Characteristic MS ions:<\/strong> [M+H]+ = 849.8 m\/z<\/li>\n<\/ul>\n<h2><strong>Purity, identification and quality control<\/strong><\/h2>\n<h3><strong>Product Specification ONE DSIP 5 mg<\/strong><\/h3>\n<p><strong>Quality parameters:<\/strong><\/p>\n<table>\n<thead>\n<tr>\n<th>Test<\/th>\n<th>Method<\/th>\n<th>Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Cleanliness<\/td>\n<td>HPLC<\/td>\n<td>\u226598%<\/td>\n<\/tr>\n<tr>\n<td>Identification<\/td>\n<td>MS<\/td>\n<td>848.81 \u00b1 0.5 Da<\/td>\n<\/tr>\n<tr>\n<td>Water content<\/td>\n<td>Karl Fischer<\/td>\n<td>\u22648%<\/td>\n<\/tr>\n<tr>\n<td>Acetate content<\/td>\n<td>Titration<\/td>\n<td>\u226415%<\/td>\n<\/tr>\n<tr>\n<td>Appearance<\/td>\n<td>Visual<\/td>\n<td>White lyophilisate<\/td>\n<\/tr>\n<tr>\n<td>Solubility<\/td>\n<td>Test<\/td>\n<td>Clear in water<\/td>\n<\/tr>\n<tr>\n<td>Net peptide content<\/td>\n<td>AA analysis<\/td>\n<td>On the certificate<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2><strong>Storage and laboratory handling<\/strong><\/h2>\n<h3><strong>Storage Conditions<\/strong><\/h3>\n<p><strong>Freeze-dried form:<\/strong><br \/>\n&#8211; Temperature: -20\u00b0C (optimal)<br \/>\n&#8211; Permissible: 2-8\u00b0C (short term)<br \/>\n&#8211; Protect from light (tryptophan sensitive to UV)<br \/>\n&#8211; Store in a dry place<br \/>\n&#8211; Shelf life: 24 months at -20\u00b0C<\/p>\n<p><strong>After reconstitution:<\/strong><br \/>\n&#8211; Solvent: sterile water or PBS<br \/>\n&#8211; Storage: 2-8\u00b0C<br \/>\n&#8211; Use within 7 days<br \/>\n&#8211; Freeze aliquots for longer storage<br \/>\n&#8211; Avoid repeated freezing\/thawing<\/p>\n<h3><strong>Reconstitution Instructions<\/strong><\/h3>\n<ol>\n<li>Equalize the temperature of the vial (15-20 min)<\/li>\n<li>Add sterile water slowly along the side<\/li>\n<li>Recommended concentration: 1-5 mg\/ml<\/li>\n<li>Mix gently by inverting the vial<\/li>\n<li>Protect the solution from light<\/li>\n<li>Store in the refrigerator or freeze<\/li>\n<\/ol>\n<h2><strong>Mechanism of action at the molecular level<\/strong><\/h2>\n<h3><strong>Molecular Interactions<\/strong><\/h3>\n<p>DSIP is studied in the context of various molecular mechanisms. It should be emphasized that the information below comes from laboratory tests and does not constitute claims of effect in humans.<\/p>\n<p><strong>Potential molecular targets (in vitro studies):<\/strong><\/p>\n<ol>\n<li><strong>Opioid receptors<\/strong><\/li>\n<\/ol>\n<ul>\n<li>Studies on the interaction with \u03bc and \u03b4 receptors<\/li>\n<li>Modulation of endorphin binding<\/li>\n<\/ul>\n<ol start=\"2\">\n<li><strong>GABA receptors<\/strong><\/li>\n<\/ol>\n<ul>\n<li>Potential modulation of GABAergic transmission<\/li>\n<li>Interactions with the GABA-A receptor complex<\/li>\n<\/ul>\n<ol start=\"3\">\n<li><strong>Glutamatergic system<\/strong><\/li>\n<\/ol>\n<ul>\n<li>Research on NMDA receptors<\/li>\n<li>Effect on excitatory transmission<\/li>\n<\/ul>\n<ol start=\"4\">\n<li><strong>Stress hormones<\/strong><\/li>\n<\/ol>\n<ul>\n<li>Interactions with the HPA axis in cellular models<\/li>\n<li>Modulation of CRH\/ACTH secretion<\/li>\n<\/ul>\n<h3><strong>Research on Cell Cultures<\/strong><\/h3>\n<p>The literature describes DSIP research on:<\/p>\n<ul>\n<li>Neurons cultured in vitro<\/li>\n<li>Pituitary cells<\/li>\n<li>Neuroblastoma cell lines<\/li>\n<li>Brain tissue preparations<\/li>\n<\/ul>\n<h3><strong>DSIP phosphorylation<\/strong><\/h3>\n<p>Serine at position 7 can be phosphorylated:<\/p>\n<ul>\n<li>pDSIP (phospho-DSIP) as the active form<\/li>\n<li>Studies on the role of phosphorylation in activity<\/li>\n<li>Phosphorylation\/dephosphorylation kinetics<\/li>\n<\/ul>\n<h2><strong>Applications in Scientific Research<\/strong><\/h2>\n<h3><strong>Neurobiology<\/strong><\/h3>\n<ul>\n<li>Study of neuropeptide mechanisms<\/li>\n<li>Modeling peptide-receptor interactions in the CNS<\/li>\n<li>Research on circadian rhythms<\/li>\n<li>Analysis of signaling pathways in neurons<\/li>\n<\/ul>\n<h3><strong>Biochemical Research<\/strong><\/h3>\n<ul>\n<li>Characteristics of binding to receptors<\/li>\n<li>Structure-activity studies<\/li>\n<li>Stability analysis in various conditions<\/li>\n<li>Peptide-membrane interactions<\/li>\n<\/ul>\n<h3><strong>Chronobiology<\/strong><\/h3>\n<ul>\n<li>Research on the biological clock<\/li>\n<li>The effect of peptides on circadian rhythms (in vitro models)<\/li>\n<li>Clock gene expression<\/li>\n<\/ul>\n<h3><strong>Comparative Research<\/strong><\/h3>\n<ul>\n<li>DSIP vs. other sleep neuropeptides<\/li>\n<li>Comparison of phosphorylated and unphosphorylated forms<\/li>\n<li>Studies of interspecies differences<\/li>\n<\/ul>\n<h3><strong>Reservations<\/strong><\/h3>\n<p><strong>IMPORTANT:<\/strong> The product is intended for in vitro testing only. Not intended for:<br \/>\n&#8211; Use in humans or animals<br \/>\n&#8211; Diagnostic or therapeutic purposes<br \/>\n&#8211; Any clinical use<\/p>\n<h2><strong>Summary<\/strong><\/h2>\n<p>DSIP (Delta Sleep Inducing Peptide) is a naturally occurring nonapeptide that is a valuable tool in neurobiological and biochemical research. Its simple structure, presence in the central nervous system of mammals, and well-understood chemical properties make it a useful reagent in neuropeptide research.<\/p>\n<p>One Peptides DSIP 5 mg material offers:<\/p>\n<ul>\n<li>High purity \u226598% HPLC<\/li>\n<li>Full spectrometric characterization<\/li>\n<li>Stable freeze-dried form<\/li>\n<li>Certificate of analysis for each batch<\/li>\n<\/ul>\n<h2><strong>Science FAQ<\/strong><\/h2>\n<h3><strong>1. What is DSIP and what is its structure?<\/strong><\/h3>\n<p>DSIP (Delta Sleep Inducing Peptide) is a nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, consisting of 9 amino acids with a molar mass of 848.81 g\/mol. It is an endogenous neuropeptide found in mammals, first isolated in 1977 from the blood of rabbits. The CAS number is 62568-57-4.<\/p>\n<h3><strong>2. Where does DSIP occur naturally?<\/strong><\/h3>\n<p>DSIP has been identified in various mammalian tissues, including the hypothalamus, pituitary gland, cerebrospinal fluid, and blood plasma. It is an endogenous neuropeptide whose presence has also been confirmed in human tissues.<\/p>\n<h3><strong>3. How to properly store DSIP?<\/strong><\/h3>\n<p>In freeze-dried form, DSIP should be stored at -20\u00b0C, protected from light (tryptophan is sensitive to UV). After reconstitution in sterile water or PBS, the solution is stored at 2-8\u00b0C and used within 7 days. For longer storage, it is recommended to freeze small portions.<\/p>\n<h3><strong>4. What is the difference between DSIP and pDSIP?<\/strong><\/h3>\n<p>pDSIP is a phosphorylated form of DSIP in which the serine residue at position 7 is phosphorylated (Ser-7-PO4). Phosphorylation may affect the biological properties of the peptide. The standard DSIP product is the unphosphorylated form.<\/p>\n<h3><strong>5. What research is DSIP used for?<\/strong><\/h3>\n<p>DSIP is used in in vitro research in the field of neurobiology, biochemistry and chronobiology. Applications include: the study of neuropeptide mechanisms, interactions with receptors in the CNS, structure-activity studies, and modeling of circadian rhythms. It is not intended for use on humans or animals.<\/p>\n<h2><strong>The most important research conclusions<\/strong><\/h2>\n<p><strong>The most important features of the ONE DSIP 5 mg product:<\/strong><\/p>\n<ul>\n<li><strong>Type<\/strong> \u2013 endogenous neuropeptide (nonapeptide)<\/li>\n<li><strong>Sequence<\/strong> \u2013 WAGGDASGE (9 amino acids)<\/li>\n<li><strong>Molar mass<\/strong> \u2013 848.81 g\/mol<\/li>\n<li><strong>CAS number<\/strong> \u2013 62568-57-4<\/li>\n<li><strong>Cleanliness<\/strong> \u2013 minimum 98% HPLC<\/li>\n<li><strong>Form<\/strong> \u2013 lyophilisate (white powder)<\/li>\n<li><strong>Property<\/strong> \u2013 contains tryptophan (fluorescent)<\/li>\n<li><strong>Destiny<\/strong> \u2013 only in vitro tests<\/li>\n<\/ul>\n<section class=\"scientific-references\">\n<h3>Bibliography<\/h3>\n<ol>\n<li><strong>Schoenenberger G. A., Monnier M. (1977)<\/strong> &#8220;Characterization of a delta-electroencephalogram (-sleep)-inducing peptide&#8221; &#8211; Proceedings of the National Academy of Sciences USA, 74(3):1282-1286.<br \/>\nLink: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC430728\/<\/li>\n<li><strong>Graf MV, Kastin AJ. (1984)<\/strong> &#8220;Delta-sleep-inducing peptide (DSIP): a review&#8221; &#8211; Neuroscience &amp; Biobehavioral Reviews, 8(1):83-93.<br \/>\nLink: https:\/\/pubmed.ncbi.nlm.nih.gov\/6145134\/<\/li>\n<li><strong>Kovalzon VM, Strekalova TV. (2006)<\/strong> &#8220;Delta sleep-inducing peptide (DSIP): a still unresolved riddle&#8221; &#8211; Journal of Neurochemistry, 97(2):303-309.<br \/>\nLink: https:\/\/pubmed.ncbi.nlm.nih.gov\/16539667\/<\/li>\n<\/ol>\n<\/section>\n<section class=\"scientific-references\">\n<h3>Chemical data source<\/h3>\n<ul>\n<li>PubChem. <a href=\"https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/68816\" target=\"_blank\" rel=\"noopener noreferrer\"><cite>Delta Sleep-Inducing Peptide, CID 68816<\/cite><\/a>.<\/li>\n<\/ul>\n<\/section>\n<p><script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is DSIP and what is its structure?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"DSIP (Delta Sleep Inducing Peptide) is a nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, consisting of 9 amino acids with a molar mass of 848.81 g\/mol. It is an endogenous neuropeptide found in mammals, first isolated in 1977 from the blood of rabbits. The CAS number is 62568-57-4.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Where does DSIP occur naturally?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"DSIP has been identified in various mammalian tissues, including the hypothalamus, pituitary gland, cerebrospinal fluid, and blood plasma. It is an endogenous neuropeptide whose presence has also been confirmed in human tissues.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How to properly store DSIP?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"In freeze-dried form, DSIP should be stored at -20\u00b0C, protected from light (tryptophan is sensitive to UV). After reconstitution in sterile water or PBS, the solution is stored at 2-8\u00b0C and used within 7 days. 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It is not intended for use on humans or animals.\"\n      }\n    }\n  ]\n}\n<\/script><\/p>\n<p><!-- onep-product-reviewer-banner:start --><\/p>\n<div class=\"author-box onep-product-reviewer-banner\" style=\"border-left: 4px solid #958e09; background: #f9fafb; padding: 16px 20px; margin: 32px 0; border-radius: 4px;\">\n<p style=\"margin: 0 0 8px 0; font-size: 14px; line-height: 1.5;\"><strong>Pharmacological review:<\/strong> <a href=\"https:\/\/one-peptides.com\/team-aneta-kropicka\/\">MPharm Aneta Kropicka<\/a><br \/>\n<em>Pharmaceutical reviewer and sports supplementation expert.<\/em><br \/>\nMaster of Pharmacy with 12 years of professional experience, graduate of the Medical University of \u0141\u00f3d\u017a (2014). Verifies One Peptides product content for pharmacology, clinical dosing, and regulatory compliance across RUO \/ dietary supplement \/ drug frameworks.<\/p>\n<p style=\"margin: 0; font-size: 12px; color: #6b7280;\"><small>Published: <time datetime=\"2024-11-23\">2024-11-23<\/time> \u2022 Last updated: <time datetime=\"2026-07-09\">2026-07-09<\/time><\/small><\/p>\n<\/div>\n<p><!-- onep-product-reviewer-banner:end --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>One Peptides DSIP 5 mg (Delta Sleep Inducing Peptide). Research Use Only (RUO). DSIP is a naturally occurring neuropeptide consisting of 9 amino acids. It is used in testing for effects on sleep.<\/p>","protected":false},"featured_media":1115,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_members_access_role":[],"_members_access_error":""},"product_brand":[],"product_cat":[81,156,71],"product_tag":[],"class_list":["post-1017","product","type-product","status-publish","has-post-thumbnail","product_cat-dsip","product_cat-longevity","product_cat-sleep","first","instock","sale","shipping-taxable","purchasable","product-type-simple"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 5.0.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"DSIP 5 mg is a RUO chemical reagent for laboratory tests. High quality. 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